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文献和实验immediately in a TA Cloning® or TOPO TA Cloning® reaction. If you wish to store your reaction, continue to Step 4. 4. Extract reaction immediately with an equal volume of phenol-chloroform. This removes all of the polymerases. 5. Precipitate the DNA
for cloning of unknown genes and analysis of expression profiling (1 -10 ). Several PCR-based strategies such as iAFLP (introduced amplified fragment length polymorphism) and TOGA (total gene-expression analysis) are currently available for global gene
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
interest in chromatin long-range interactions. RELATED INFORMATION The 6C assay combines three different methodologies: chromosome conformation capture (3C) (Dekker et al. 2002), chromatin immunoprecipitation (ChIP), and cloning (Fig. 1 ). The first step
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