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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Taq DNA Polymwease(含10*Taq Buffer,Mg+)
- 供应商:
南京赛泓瑞
- 规格:
500u,2.5u/ul
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文献和实验Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
Steve HahnProtein Dilution Buffer 5ml20 mM Tris pH7.9 100 microliters 1M Tris 7.9150 mM KCl 0.75 ml 1 M KCl1 mM DTT 50 microliters 0.1 M DTT10% glycerol 1 ml 50% glycerol50 micrograms/ml BSA 2.5 microliters 100 mg/ml BSA3.1 ml H2OOptional: add Brij
of a master mix containing water, buffer, dNTPs, primers and Taq DNA Polymerase in a single tube, which can then be aliquoted into individual tubes. MgCl2 and template DNA solutions are then added. This method of setting reactions minimizes the possibility
ml 1M Tris pH 8.5*50 mM dextrose 4.5 g dextrose1 mM EDTA 1 ml 0.5M EDTAup to 500 ml with Q*Check pH and bring to 7.9 as the dextrose will reduce the pH.Store at room temperature.For some steps add Lysozyme to afinal concentration of 4 mg/ml.Buffer B10
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