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Sigma货号D2650-100ml二甲基亚砜DMSO上海睿

安生物13611631389
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  • Sigma
  • Made in USA
  • D2650
  • 2025年10月07日
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    • 文献和实验
    • 技术资料
    • 保存条件：

      Room temperature

    • 保质期：

      1-2年

    • 英文名：

      DMSO

    • 库存：

      100⁺瓶

    • 供应商：

      上海睿安生物13611631389

    • 规格：

      100ml/瓶

    Sigma货号D2650-100ml二甲基亚砜DMSO

    上海睿安生物13611631389

    说明

    应用

    DMSO是一种用于化学反应[如聚合酶链式反应(PCR)]的极性非质子溶剂，也可作为冷冻保护玻璃化剂用于保存细胞、组织和器官。DMSO可在细胞冷冻培养基中用于保护细胞免受冰晶导致的机械损伤。它可用于原代、继代培养和重组的异倍体和杂交瘤细胞系、胚胎干细胞(ESC)以及造血干细胞的冻存。DMSO常与BSA或胎牛血清(FBS)一同使用。

    包装

    5ml和10ml火焰封口安瓿瓶包装，100ml棕色瓶包装
    Glass ampoule packaging is now available under a new product number,D2653

    其他说明

    该产品是一种Hybri-Max产品。它经过了杂交瘤测试并对其在细胞冷冻中的适用性进行了评估。该产品经无菌过滤(0.2微米)及内毒素水平检测。
    2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

    注意:在室温下容易过冷并缓慢重融。 固化产品可以通过加热至室温来重新液化，而不会损害产品。

    常见问题

    1. What is the molarity of DMSO?

      DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

    2. What is the DMSO dissolved in?

      DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.

    3. Where does DMSO come from?

      DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

    4. What concentration of DMSO do I use in a cell freezing media?

      DMSO is usually used at 1-10%, depending on the cell line.  For the cell freezing media that we offer, it is used at 8.7% concentration.

    5. Does DMSO have any synthetic utility?

      DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

    6. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    7. What is the specification for 'pass' for cell freezing on the Certificate of Analysis for Dimethyl sulfoxide (DMSO)?

      Product D2438 is assessed for cell freezing.  Cells are frozen and stored at -70°C.  The cells are thawed, cultured and viability is assessed at 24 hours.  There is no evidence of cytotoxicity or abnormal cell growth.

    8. Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?

      DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.

    What is the basis for the endotoxin specification for Dimethyl sulfoxide (DMSO)?

    The industry standard for 'endotoxin free' is less than 1 EU/mL.

    What type of filter is used to sterile-filter Dimethyl sulfoxide (DMSO)?

    DMSO can be sterile-filtered through a 0.2 micron PTFE filter. The sterility testing is per the current US Pharmacopoeia method.

    How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

    同行评审论文(部分文献)

    Preparation of hydrophilic polycaprolactone/modified ZIF-8 nanofibers as a wound dressing using hydrophilic surface modifying macromolecules.
    Azadeh Ghaee et al.
    Materials science & engineering. C, Materials for biological applications, 103, 109767-109767 (2019-07-28)
    In this study, a simple one-step method was introduced to prepare suitable PCL wound dressing by using a small amount of hydrophilic surface modifying macromolecule (LSMM) with ends capped with Polyethylene glycol and aminated zeolitic imidazolate frameworks (MZIF-8) as a
    Inflammatory Bowel Disease Types Differ in Markers of Inflammation, Gut Barrier and in Specific Anti-Bacterial Response.
    Stepan Coufal et al.
    Cells, 8(7) (2019-07-25)
    Crohn's disease (CD), ulcerative colitis (UC) and inflammatory bowel disease (IBD) associated with primary sclerosing cholangitis (PSC-IBD), share three major pathogenetic mechanisms of inflammatory bowel disease (IBD)-gut dysbiosis, gut barrier failure and immune system dysregulation. While clinical differences among them
    Paeonol induces cytoprotective autophagy via blocking the Akt/mTOR pathway in ovarian cancer cells.
    Likun Gao et al.
    Cell death & disease, 10(8), 609-609 (2019-08-14)
    Paeonol (Pae), a phenolic acid compound isolated from the Moutan Cortex, was previously demonstrated to exert multiple anticancer effects. The rational control of autophagy has been considered a potential treatment strategy for epithelial ovarian cancer. However, whether Pae induces autophagy
    Ivermectin inhibits canine mammary tumor growth by regulating cell cycle progression and WNT signaling.
    Hongxiu Diao et al.
    BMC veterinary research, 15(1), 276-276 (2019-08-04)
    Mammary gland tumor is the most common spontaneous tumor in intact female dogs, and its poor prognosis remains a clinical challenge. Ivermectin, a well-known anti-parasitic agent, has been implicated as a potential anticancer agent in various types of human cancer.
    CRISPR/Cas9 as a tool to dissect cancer mutations.
    Shady Sayed et al.
    Methods (San Diego, Calif.), 164-165, 36-48 (2019-05-13)
    The CRISPR/Cas9 system is transforming many biomedical disciplines, including cancer research. Through its flexible programmability and efficiency to induce DNA double strand breaks it has become straightforward to introduce cancer mutations into cells in vitro and/or in vivo. However, not

    产品细节图片1产品细节图片2产品细节图片3产品细节图片4产品细节图片5
    产品细节图片6
    产品细节图片7

    现货供应Sigma货号D2650-100ml二甲基亚砜(别名DMSO)询订13611631389上海睿安生物
     
    一、╳※试剂※╳:
    常规血清优势供应:VivaCell货号C04001-500血清、Gemini货号900-108血清、Gemini货号100-512血清、Gibco血清(10099-141澳洲血清、10270-106南美血清、16000-044血清)、BI血清BI货号04-001-1ACS、Sigma货号F8318-500ml澳洲胎牛血清、Sigma货号F8687澳洲胎牛血清、Sigma货号F2442-500ml特级胎牛血清、ScienCell货号0500澳洲胎牛血清、Cegrogen货号A0500-3010胎牛血清、Cegrogen货号B0500-5010新生牛血清、康源血清
    培养基(包括无血清)、冻存液(包括无血清)，包括:友康Yocon
    Merck(Millipore/Sigma/Roche/KaPa/Supelco/Avanti/Corning/Brand)正式授权代理、博奥龙Biodragon独家总代理、EFL独家总代理、碧云天Beyotime代理
    ☆☆☆☆☆☆Beckman货号A63881纯化磁珠Agencourt AMPure XP(60ml/瓶)/A63882(450ml/瓶)；Beckman货号B23317核酸片段筛选试剂盒Agencourt SPRIselect(5ml/瓶)
    分子类:Biotium货号41003的GelRed染料、货号31000的EvaGreen染料、Zymo Research、Qiagen
    饲料类:Research Diets、Dyets
    二、╳※耗材※╳:
    洁特JET耗材(各类常规用实验耗材)、赛普Cellpro耗材/KirGen耗材、博麦迪(常规的)各类试剂瓶
    Bioplastics
    Thermo Fisher Scientific的很多产品线，包括旗下的:
    一、Nunc耗材
    二、QSP耗材(各类吸头/滤芯吸头、离心管、PCR管等)
    三、Nalgene耗材仪器
    四、FisherBrand耗材仪器(含各类盖玻片载玻片等)
    五、Lab_Serv耗材仪器
    六、Finnpipette移液器(如：F1系列、F2系列、F3系列等等各类型号的移液器)
    七、Abgene耗材
    畅销款:我们是常备有大量现货的
    可请随时咨询:万奎13611631389(可以请加微信)
    产品细节图片8
    产品细节图片9

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    • 内容
    • 询问日期
    图标文献和实验
    该产品被引用文献

    Sigma货号D2650-100ml二甲基亚砜DMSO

    上海睿安生物13611631389

    说明

    应用

    DMSO是一种用于化学反应[如聚合酶链式反应(PCR)]的极性非质子溶剂，也可作为冷冻保护玻璃化剂用于保存细胞、组织和器官。DMSO可在细胞冷冻培养基中用于保护细胞免受冰晶导致的机械损伤。它可用于原代、继代培养和重组的异倍体和杂交瘤细胞系、胚胎干细胞(ESC)以及造血干细胞的冻存。DMSO常与BSA或胎牛血清(FBS)一同使用。

    包装

    5ml和10ml火焰封口安瓿瓶包装，100ml棕色瓶包装
    Glass ampoule packaging is now available under a new product number,D2653

    其他说明

    该产品是一种Hybri-Max产品。它经过了杂交瘤测试并对其在细胞冷冻中的适用性进行了评估。该产品经无菌过滤(0.2微米)及内毒素水平检测。
    2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

    注意:在室温下容易过冷并缓慢重融。 固化产品可以通过加热至室温来重新液化，而不会损害产品。

    常见问题

    1. What is the molarity of DMSO?

      DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

    2. What is the DMSO dissolved in?

      DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.

    3. Where does DMSO come from?

      DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

    4. What concentration of DMSO do I use in a cell freezing media?

      DMSO is usually used at 1-10%, depending on the cell line.  For the cell freezing media that we offer, it is used at 8.7% concentration.

    5. Does DMSO have any synthetic utility?

      DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

    6. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    7. What is the specification for 'pass' for cell freezing on the Certificate of Analysis for Dimethyl sulfoxide (DMSO)?

      Product D2438 is assessed for cell freezing.  Cells are frozen and stored at -70°C.  The cells are thawed, cultured and viability is assessed at 24 hours.  There is no evidence of cytotoxicity or abnormal cell growth.

    8. Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?

      DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.

    What is the basis for the endotoxin specification for Dimethyl sulfoxide (DMSO)?

    The industry standard for 'endotoxin free' is less than 1 EU/mL.

    What type of filter is used to sterile-filter Dimethyl sulfoxide (DMSO)?

    DMSO can be sterile-filtered through a 0.2 micron PTFE filter. The sterility testing is per the current US Pharmacopoeia method.

    How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

    同行评审论文(部分文献)

    Preparation of hydrophilic polycaprolactone/modified ZIF-8 nanofibers as a wound dressing using hydrophilic surface modifying macromolecules.
    Azadeh Ghaee et al.
    Materials science & engineering. C, Materials for biological applications, 103, 109767-109767 (2019-07-28)
    In this study, a simple one-step method was introduced to prepare suitable PCL wound dressing by using a small amount of hydrophilic surface modifying macromolecule (LSMM) with ends capped with Polyethylene glycol and aminated zeolitic imidazolate frameworks (MZIF-8) as a
    Inflammatory Bowel Disease Types Differ in Markers of Inflammation, Gut Barrier and in Specific Anti-Bacterial Response.
    Stepan Coufal et al.
    Cells, 8(7) (2019-07-25)
    Crohn's disease (CD), ulcerative colitis (UC) and inflammatory bowel disease (IBD) associated with primary sclerosing cholangitis (PSC-IBD), share three major pathogenetic mechanisms of inflammatory bowel disease (IBD)-gut dysbiosis, gut barrier failure and immune system dysregulation. While clinical differences among them
    Paeonol induces cytoprotective autophagy via blocking the Akt/mTOR pathway in ovarian cancer cells.
    Likun Gao et al.
    Cell death & disease, 10(8), 609-609 (2019-08-14)
    Paeonol (Pae), a phenolic acid compound isolated from the Moutan Cortex, was previously demonstrated to exert multiple anticancer effects. The rational control of autophagy has been considered a potential treatment strategy for epithelial ovarian cancer. However, whether Pae induces autophagy
    Ivermectin inhibits canine mammary tumor growth by regulating cell cycle progression and WNT signaling.
    Hongxiu Diao et al.
    BMC veterinary research, 15(1), 276-276 (2019-08-04)
    Mammary gland tumor is the most common spontaneous tumor in intact female dogs, and its poor prognosis remains a clinical challenge. Ivermectin, a well-known anti-parasitic agent, has been implicated as a potential anticancer agent in various types of human cancer.
    CRISPR/Cas9 as a tool to dissect cancer mutations.
    Shady Sayed et al.
    Methods (San Diego, Calif.), 164-165, 36-48 (2019-05-13)
    The CRISPR/Cas9 system is transforming many biomedical disciplines, including cancer research. Through its flexible programmability and efficiency to induce DNA double strand breaks it has become straightforward to introduce cancer mutations into cells in vitro and/or in vivo. However, not

    引用文献图片1引用文献图片2引用文献图片3引用文献图片4引用文献图片5
    引用文献图片6
    引用文献图片7

    现货供应Sigma货号D2650-100ml二甲基亚砜(别名DMSO)询订13611631389上海睿安生物
     
    一、╳※试剂※╳:
    常规血清优势供应:VivaCell货号C04001-500血清、Gemini货号900-108血清、Gemini货号100-512血清、Gibco血清(10099-141澳洲血清、10270-106南美血清、16000-044血清)、BI血清BI货号04-001-1ACS、Sigma货号F8318-500ml澳洲胎牛血清、Sigma货号F8687澳洲胎牛血清、Sigma货号F2442-500ml特级胎牛血清、ScienCell货号0500澳洲胎牛血清、Cegrogen货号A0500-3010胎牛血清、Cegrogen货号B0500-5010新生牛血清、康源血清
    培养基(包括无血清)、冻存液(包括无血清)，包括:友康Yocon
    Merck(Millipore/Sigma/Roche/KaPa/Supelco/Avanti/Corning/Brand)正式授权代理、博奥龙Biodragon独家总代理、EFL独家总代理、碧云天Beyotime代理
    ☆☆☆☆☆☆Beckman货号A63881纯化磁珠Agencourt AMPure XP(60ml/瓶)/A63882(450ml/瓶)；Beckman货号B23317核酸片段筛选试剂盒Agencourt SPRIselect(5ml/瓶)
    分子类:Biotium货号41003的GelRed染料、货号31000的EvaGreen染料、Zymo Research、Qiagen
    饲料类:Research Diets、Dyets
    二、╳※耗材※╳:
    洁特JET耗材(各类常规用实验耗材)、赛普Cellpro耗材/KirGen耗材、博麦迪(常规的)各类试剂瓶
    Bioplastics
    Thermo Fisher Scientific的很多产品线，包括旗下的:
    一、Nunc耗材
    二、QSP耗材(各类吸头/滤芯吸头、离心管、PCR管等)
    三、Nalgene耗材仪器
    四、FisherBrand耗材仪器(含各类盖玻片载玻片等)
    五、Lab_Serv耗材仪器
    六、Finnpipette移液器(如：F1系列、F2系列、F3系列等等各类型号的移液器)
    七、Abgene耗材
    畅销款:我们是常备有大量现货的
    可请随时咨询:万奎13611631389(可以请加微信)
    引用文献图片8
    引用文献图片9
    相关实验

    • Cryopreservation of Cell Lines

      controlled freezer is recommended. Materials Freeze medium (commonly 70% basal medium, 20% FCS, 10% DMSO (Prod. No. D2650 ) or glycerol, check ECACC data sheets for details). 70% ethanol in water (Prod. No. R8382 ) PBS without Ca

    • 细胞冻存、解冻方法与细胞计数

      之状态，约为80～90％致密度。冷冻前检测细胞是否仍保有其特有性质，例如hybridoma应在冷冻保存前一至二日测试是否有抗体之产生。（2）细胞在液氮中可长期冻存无限时间，而不会影响细胞活力；在-70度可保存数月。（3）注意冷冻保护剂之品质。DMSO应为试剂级等级，无菌且无色(以0.22micron　FGLP　Telflon过滤或是直接购买无菌产品，如Sigma D-2650)，以5～10ml小体积分装，4℃避光保存，勿作多次解冻。Glycerol亦应为试剂级等级，以高压蒸汽灭菌后避光保存。在开启

    • 细胞解冻的实验流程和几点需知

      其特有性质，例如hybridoma应在冷冻保存前一至二日测试是否有抗体之产生。 (2)细胞在液氮中可长期冻存无限时间，而不会影响细胞活力;在-70度可保存数月。 (3)注意冷冻保护剂之品质。DMSO 应为试剂级等级，无菌且无色(以0.22micron　FGLP　Telflon过滤或是直接购买无菌产品，如Sigma D-2650)，以5～10ml小体积分装，4℃避光保存，勿作多次解冻。Glycerol亦应为试剂级等级，以高压蒸汽灭菌后避光保存。在开启后一年内使用，因长期储存

    查看更多相关实验 >

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