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文献和实验Measurement of Oxygen Radicals and Lipid Peroxidation in Neural Tissues
species (RNS). These are generated by multiple mechanisms and give rise to highly reactive oxygen radicals that can damage neuronal, glial, and microvascular elements. Particular interest has centered upon oxygen radical?induced, iron?catalyzed lipid
-seedling proteins. Electrophoresis7, 52-54. 10 . Granier, F. (1988) Extraction of plant proteins for two-dimensional electrophoresis. Electrophoresis 9,71 2-718. 11. Islam, N., Tsujimoto, H., and Hirano, H. (2003) Proteome analysis
via a basic Bligh-Dyer extraction: a) Resuspend cell pellet in 3 ml chloroform/methanol (1:2) & vortex. b) Add 0.8 ml water & vortex. c) Pellet cellular debris by spinning at 3,000 rpm (2000xg) for 5 min. d) Add 1 ml chloroform & vortex. e) Add 1 ml water
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