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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
南京赛泓瑞生物
- 检测范围:
0.312-20 ng/mL
- 检测方法:
Elisa
- 应用:
Elisa
- 适应物种:
Homo sapiens (Human)
- 样本:
Homo sapiens (Human)
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | 询价 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | 询价 |
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
, should be used. 2. Add 80 ul Buffer DS and vortex to mix. 3. Incubate at 70°C for 10 min. Briefly vortex the tube once during the incubation. For some difficult lysis bacterial, Increase the temperature to 90°C. 4. Centrifuge at 13,000 x g
, ON for 48 hours. filter on 0.22 µm filter. run ELISA to establish the Ig concentration SOLUTIONS ammonium sulphate, saturated, pH 6.8, store at RT PBS ѓ [ p u r
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