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- 2025年07月13日
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文献和实验, 50 µg streptomycin and 50 µM 2-ME in the absence or presence of HAT or HT for selection (I-20). Procedure: NOTE: All washes are at 1,000 rpm for 10 minutes at 4° C using serum free media Tissue Collection: 1. Sacrifice animal
General Fusion Protocol Materials: P3X63Ag8.653 murine myeloma or YB2/0 (maintained at 6 /ml) 50% w/v PEG 1500, warmed to 37° C Medium: IMDM supplemented with 20% fetal bovine serum, 4 mM L-glutamine
PriCells: Methods of Isolation and Culture of Human Hepatocytes
perfusion modified from the method, which was derived from the method. The major modifications were the substitution of EBSS for HEPES as the perfusion buffer and an additional washing step to remove residual EGTA prior to collagenase perfusion, adopted
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