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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
6 months
- 英文名：
SuperSepTM Phos-tagTM (50μmol/l), 12.5%, 13well
- 供应商：
富士胶片和光
- 保存条件：
冷藏
- 规格：
5块
This is a precast gel added with Phos-tag™ Acrylamide in advance. It can be used immediately after opening the package. It contains zinc as a supplemental metal. It has excellent storage stability by its neutral gel buffer. Sharp bands can be obtained.
Features:
- Phosphorylated- and non-phonphorylated proteins are efficiently separable with sharp bands.
- Long term stability (stable for 6 months)
- Plate size: 100 x 100 x 3 (mm)
- Gel size: 90 x 85 x 1 (mm)
- Well number: 13
- Well volume: 30 μL
- Please note that prestain marker does not reflect the molecular weight, when a gel containing Phos-tag™ is use. It can only be used as reference of electrophoresis. Please use the result as an index of transcription efficiency at Western blotting. At Western blotting, Zn2+ must be sufficiently removed with transfer buffer containing 10 mM EDTA. Please wash out the gel for 10 minutes x 3 times. Subsequently, perform the same procedure for 10 minutes using transfer buffer without EDTA, then transfer the gel.
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
background. 4. Incubate tissue in X-Phos/NBT Detection Buffer for 15' at room temperature. XPhos/NBT Detection Buffer (Buffer 3 as described for Genius kit by Boehringer-Mannheim) 100 mM Tris-HCl, pH 9.5 100 mM NaCl 50 mM MgCl
/NaCl: 20% PEG-8000 ( m/V ) 和 2.5 mol/L 氯化钠；高压灭菌。 ( 10 ) QIA prep Spin M13 试剂盒（Qiagen，Valencia，美国加州）。 ( 11 ) M13KO7 辅助噬菌体（NEB，Beverly，美国麻省）。 ( 12 ) Tris- acetate-EDIA (TAE) 缓冲液：40 mmol/L Tris-acetate 和 1 mmol/L EDTA；调节 pH 到 8.0；高压灭菌。 ( 13
于链霉抗生物素包被的微孔板上（图10）。 1.4.3 将SNAP-tagTM用于蛋白固相化和纯化 传统的蛋白纯化方法是基于标签蛋白与纯化基质之间不稳定的相互作用（亲和力），这就导致很多的融合蛋白由于结合力不够，没有结合在基质，损失了蛋白产量。此外，由于不能够用很严谨的条件进行清洗，又导致了很高的背景。而SNAP-tagTM用于纯化则可克服上述缺点（图11、12）。 1.4.4 将SNAP-tagTM用于活细胞中融合蛋白的标记 将SNAP-tagTM用于活
