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- 文献和实验
- 技术资料
- 库存:
100/box
- 英文名:
2 RECTANGULAR WELL MULTIWELL SLIDES, 25 X 20 mm RECT.
- 保存条件:
ROOM TEMPERATURE
- 供应商:
西宝生物
- 规格:
LABWARE
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文献和实验Multiwell Transformation Protocol
Multiwell Transformation Protocol Labor-saving implements Multichannel pipettors--1 to 25 µl and 20 to 200 µl volume (Brinkmann 12-channel). Sterile 96-well assay or tissue culture plate with lid
Staining protocols for placental explant cultures
into a rectangular shape about 4 x 8 mm, ensuring that one short edge is cut at right angles across the explant growth. This will be the face that is sectioned and the maximum area of outgrowth possible is desirable. Transfer to a glass vial for processing. Post-fix
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
for 2 days. 10. Rinse slides in PBS + 20 mM EDTA, 3x 10 minutes. 11. Mount in Gelvatol (+ 20 mM EDTA if desired). Storing slides at -80o C helped prevent background staining from increasing. Notes If cultured cells
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