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- 文献和实验
- 技术资料
- 保存条件:
-70 C
- 供应商:
西宝生物
- 英文名:
TUBULIN
- 库存:
5 mg
- 规格:
IMMUNOBIOLOGICALS
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文献和实验SDS Gel Electrophoresis of Tubulin\MAPs
. Scan the gels or photographically analyze them. Determine the molecular weights of the proteins (tubulin and MAP's).
I. Useful Values 1 mg/ml tubulin = 10 µM (assuming MW of ab-tubulin heterodimer is 100,000; in reality it is ~110,000 but almost all tubulin labs use this convenient conversion relationship) 1 µm of a microtubule has ~1600
remove the supernatants from the pellets, which are sometimes loose, and pool in a graduated cylinder. Note the volume (V2). 16. Add an equal volume of PM-8M, mix gently and store at -20°C. The tubulin at this stage
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