TUBULIN产品图

TUBULIN

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  • 询价
  • MP
  • 08771122
  • 进口
  • 2025年07月10日
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    • 文献和实验
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    • 保存条件:

      -70 C

    • 供应商:

      西宝生物

    • 英文名:

      TUBULIN

    • 库存:

      5 mg

    • 规格:

      IMMUNOBIOLOGICALS

    Purified bovine brain tubulin protein supplied at 10 mg/ml in 80 mM PIPES buffer containing 1 mM EGTA, 1 mM GTP, and 10% glycerol. It is a heterodimer composed of a - and b -tubulin of 55 kDa. Polymerization occurs forming microtubules of 25 nm in diameter and average 1650 heterodimers per m m in length. Unit Definition: One unit is defined as 5.0 mg of purified protein (Bradford). Tubulin at a concentration of 1.0 unit/ml incubated at 35 <144> C for 30 minutes will increase in absorbance at 340 nm from 0 to 0.650, corresponding to a microtubule mass of 3.6 mg/ml. The mean length of the microtubule population under these conditions is approximately 10 m m, as measured by immunofluorescence microscopy, and the average number of microtubules/ml is 1.0 x 10<^>12 .

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    图标文献和实验
    相关实验
    • SDS Gel Electrophoresis of Tubulin\MAPs

      .   Scan the gels or photographically analyze them.   Determine the molecular weights of the proteins (tubulin and MAP's).  

    • Tubulin Basics

        I. Useful Values 1 mg/ml tubulin = 10 µM (assuming MW of ab-tubulin heterodimer is 100,000; in reality it is ~110,000 but almost all tubulin labs use this convenient conversion relationship) 1 µm of a microtubule has ~1600

    • Tubulin Preparat

      remove the supernatants from the pellets, which are sometimes loose, and pool in a graduated cylinder. Note the volume (V2). 16. Add an equal volume of PM-8M, mix gently and store at -20°C. The tubulin at this stage

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