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c-Myc MONOCLONAL ANTIBODY
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
TRIS-(2,3-DIBROMOPROPYL)PHOSPHATE
- 供应商：
西宝生物
- 库存：
100g
- 保存条件：
Invalid
- 规格：
BIOCHEMICALS
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文献和实验Steady State ATPase Assays Coupled Enzyme System
, in Tris-HCl. Avoid using phosphate buffers for ATPase assays. 2. The coupled enzyme ATPase assay is based on the conversion of phosphoenolpyruvate (PEP) to pyruvate by pyruvate kinase (PK) coupled to the conversion
Protein G Purification of Antibodies
1. Reagent and Materials(1) Hi-Trap Protein G Column (Pharmacia Biotech #17-0404-01)(2) 20 mM Sodium Phosphate Buffer, pH 7.01.084 g NaH2PO4, anhydrous3.273 g Na2HPO4.7H2Oq.s. to 1 liter with di-H2O(3) 0.1M Glycine, pH 2.83.75 g Glycine1.4 ml HCl
Alkaline Southern Blotting Procedure
. Remove the membrane and rinse in 200ml: 0.1M Tris Buffer pH 7 15 min. 1.0M NaCl Blot dry on 3mm paper. Bake in 80 C vacuum oven for 90 min. PREHYBE SOLUTION: 2% SDS 0.25 M Na Phosphate buffer pH 7.2 0.5 mg/ml Herring Sperm DNA HYBE SOLUTION
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