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- 文献和实验
- 技术资料
- 抗体英文名:
Anti-Mouse CD34 FITC
- 规格:
50ug
It has been reported that the RAM34 antibody can be used to detect CD34+Sca-1+c-Kit+ cells.
CD34 is a highly glycosylated (approximately 90-120 kDa) member of the sialomucin family and is
expressed by capillary endothelial cells, bone marrow stroma, and a small subpopulation of
mouse bone marrow cells. RAM34 has been used to purify mouse hematopoietic stem cells (HSC)
to near homogeneity. CD34 expressed on endothelial cells is a ligand for CD62L and plays a
role in adhesion. Simultaneous staining of mouse bone marrow cells with a cocktail of antibodies
to lineage markers (CD3, CD11b, Ly6G, TER-119 and CD45R/B220) reveals a subset of cells that
stain with the RAM34 antibody and express undetectable to low levels of the indicated lineage markers.
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectin Culture medium PBS Fixative: 4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit
FITC荧光二抗?――EarthOx新型DyLight 488绿色荧光
中英文品名 品牌 货号 规格 详情 DyLight 488 AffiniPure Goat Anti-Mouse IgG(H+L) EarthOx E032210 ¥200/100μl ¥800/500μl 详情 DyLight 488
与在各种检测方案中所应用的不同技术。并就外周血干细胞计数标准方案达成了一致。推荐方案中要求标本需预先作白细胞计数,用来染色的标本体积根据对 CD34+ 细胞数量估算来决定,分析时要求收集足够的有统计学意义的标本数量。方案中推荐使用双标记方法染色,其中包括将 2 ul 的 PE 标记的 CD3 抗体和 10ul 的 FITC 标记的 CD34 抗体( QBEnd10 )加入 250ul 的全血进行染色,室温下孵育 20 分钟,每 5 分钟用移液器吹打混匀一次;随后加入 Ortho 公司的红细胞裂解液孵育
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