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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
Human Immunoglobulin Master Buffer Kit
- 规格:
100T
Human Immunoglobulin Master Buffer Kit
BD Cytometric Bead Array (CBA)
Contents
Assay diluent, capture bead diluent, detection reagent diluent, wash buffer, instrument setup reagents
Application
Flow cytometry (Routinely Tested)
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文献和实验samples, MPC-15 for 1–15 ml samples and MPC-50 for 15–50 ml samples. Mixer allowing both tilting and rotation. Buffer 1: PBS (without Ca2 and Mg2 ) w/0.1% BSA and 2 mM EDTA, pH 7.4. Buffer 2: PBS pH 7.4 (without Ca2 and Mg2 ).
2) approximately 10 mg FFPE sample add 95 ul FP1 Buffer and 5 μl FP2 to the collection tube. Close the tube and vortex briefly. Make sure the sample is covered by the Extraction Solution. 3) Incubate at 60°C for 30-60 minutes. 4) Incubate at 90°C
water for every mg of transferred ground seed material into the sample tube and vortex to mix. d. Continue to Extract seeds DNA 4) Extract of ground seeds DNA a. Add 45 μl PS1 Buffer and 5 μl PS2 Buffer
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