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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Anti-Human CD16 FITC
- 规格：
25tests
for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two
similar genes, CD16A (Fc gammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored
form in macrophages and NK cells and CD16B (Fc gammaRIIIB), which exist as a monomeric
GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B,
NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared
with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding
of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding,
both CD16 isoforms initiate signal transduction cascades that lead to a variety of
responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis,
degranulation and proliferation.
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectin Culture medium PBS Fixative: 4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit
FITC荧光二抗？――EarthOx新型DyLight 488绿色荧光
FITC荧光二抗？――EarthOx新型DyLight 488绿色荧光 FITC（Fluorescein Isothiocyanate，异硫*酸荧光素）是一种绿色荧光团，在免疫学实验里，经常被用于荧光团标记到相应的检测分子，如抗体上。FITC的纯品为黄色或橙黄色结晶粉末，易溶于水和酒精溶剂。FITC分子量为389.4，最大吸收光波长为 490～495nm，最大发射光波长为520～530nm，呈现明亮的黄绿色荧光。FITC在冷暗干燥处可保存多年，是目前应用最广泛的荧
Immunohistochemistry using Anti-Ganglioside Antibodies
supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab
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