人乙型肝炎核心抗原单克隆抗体

英文名称 HBcAg(1H8)
中文名称 人乙型肝炎核心抗原单克隆抗体
别 名 C; Capsid protein; Core and e antigen; Core antigen; Core antigen; Core protein; HBc; HBcAg; HBVgp4; Hepatitis B Virus core antigen; p21.5; Hepatitis B Virus Core Antigen; precore/core protein; CAPSD_HBVA3.
人乙型肝炎核心抗原单克隆抗体
说 明 书 0.1ml 0.2ml
研究领域 免疫学 细菌及病毒
抗体来源 Mouse
克隆类型 Monoclonal
克 隆 号 1H8
交叉反应 HBV
产品应用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 21kDa
性 状 Lyophilized or Liquid
浓 度 1mg/1ml
免 疫 原 Recombinded human hepatitis B core antigen protein
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
人乙型肝炎核心抗原单克隆抗体
PubMed PubMed
产品介绍 background:
Hepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection.
The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection.

Function:
Self assembles to form an icosahedral capsid. Mostcapsid appear to be large particles with a icosahedral symmetry ofT=4 and consist of 240 copies of capsid protein, though a fractionforms smaller T=3 particles consisting of 180 capsid proteins.Entering capsid are transported along microtubules to the nucleus.Phosphorylation of the capsid is thought to induce exposure ofnuclear localization signal in the C-terminal portion of the capsidprotein that allows binding to the nuclear pore complex via theimportin (karyopherin-) alpha and beta. Capsids are imported inintact form through the nuclear pore into the nuclear basket, whereit probably binds NUP153. Only capsids that contain the matureviral genome can release the viral DNA and capsid protein into thenucleoplasm. Immature capsids get stucked in the basket. Capsidsencapsulate the pre-genomic RNA and the P protein. Pre-genomic RNAis reverse transcribed into DNA while the capsid is still in thecytoplasm. The capsid can then either be directed to the nucleus,providing more genome for transcription, or bud through theendoplasmic reticulum to provide new virions (By similarity).
Encapsidates hepatitis delta genome.
人乙型肝炎核心抗原单克隆抗体