3-磷酸甘油醛脱氢酶单克隆抗体(内参抗体)

英文名称 GAPDH(3E12)-Loading Control
中文名称 3-磷酸甘油醛脱氢酶单克隆抗体(内参抗体)
别 名 38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase;Glyceraldehyde 3 phosphate dehydrogenase liver;Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10.
3-磷酸甘油醛脱氢酶单克隆抗体(内参抗体)
说 明 书 0.1ml 0.2ml
产品类型 内参抗体
研究领域 免疫学
抗体来源 Mouse
克隆类型 Monoclonal
克 隆 号 3E12
交叉反应 Mouse, Rat, Rabbit,
产品应用 WB=1:100-1000 ELISA=1:1000-5000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 38kDa
细胞定位 细胞核 细胞浆 细胞膜
性 状 Lyophilized or Liquid
浓 度 1mg/1ml
免 疫 原 full length GAPDH protein
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存条件 Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20癈. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 癈.
3-磷酸甘油醛脱氢酶单克隆抗体(内参抗体)
产品介绍 background:
Loading Control
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.

Function:
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Subunit:
Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with EIF1AD, USP25, PRKCI and WARS.

Subcellular Location:
Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions.

Post-translational modifications:
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated (Probable).
Sulfhydration at Cys-152 increases catalytic activity.

Similarity:
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
3-磷酸甘油醛脱氢酶单克隆抗体(内参抗体)
xy-4611R Aquaporine 2水通道蛋白-2抗体
xy-5984R ABI2肿瘤抑制基因ABI2/精氨酸酪氨酸蛋白激酶结合蛋白抗体
xy-5987R ARL11ADP核糖基化因子样11抗体
xy-5990R ARMCX3ARM重复X连锁蛋白ARMCX3抗体
xy-5988R ARMCX1ARM重复X连锁蛋白ARMCX1抗体
xy-5989R ARMCX2ARM重复X连锁蛋白ARMCX2抗体
xy-5991R Apg10自噬相关蛋白10抗体
xy-6017R ACBD6酰基辅酶A结合结构域蛋白6抗体
xy-6019R Aminoacylase 1氨基酰化酶1抗体
xy-3831R Adipose Triglyceride Lipase脂肪甘油三酯脂酶抗体
xy-4002R phospho-AMPK alpha-1 (Thr172)磷酸化腺苷单磷酸活化蛋白激酶α1抗体
xy-3847R Amphiregulin双调蛋白（结肠直肠细胞源性生长因子）抗体
xy-5185R phospho-AQP2(Ser264)磷酸化水通道蛋白2抗体
xy-5180R AXL粘附相关激酶抗体
xy-5181R phospho-AXL (Tyr698+Tyr702+Tyr703)磷酸化粘附相关激酶抗体
xy-5182R phospho-AKT1 + AKT2 + AKT3 (Thr308+Thr309+Thr305)磷酸化蛋白激酶B抗体
xy-5187R phospho-AQP2(Ser269)磷酸化水通道蛋白2抗体
xy-5186R phospho-Amyloid Precursor Protein (Thr743)磷酸化APP淀粉样肽前体蛋白抗体
xy-5188R phospho-AKT1(Ser129)磷酸化蛋白激酶B抗体
xy-5189R phospho-Amyloid Precursor Protein (Ser198)磷酸化APP淀粉样肽前体蛋白抗体
xy-5190R phospho-ATF4 (Ser245)磷酸化活化转录因子4抗体
xy-5191R phospho-Androgen Receptor (Ser213)磷酸化雄激素受体抗体
xy-5192R phospho-Androgen Receptor (Ser650)磷酸化雄激素受体抗体
xy-5193R phospho-AKT1+2+3 (Tyr315+316+312) 磷酸化蛋白激酶AKT1,2,3抗体
xy-5194R phospho-AKT1(Thr34)磷酸化蛋白激酶B抗体
xy-5195R phospho-ATF2(Ser472)磷酸化活化复制因子2抗体
xy-9806R APR3凋亡相关蛋白3抗体
xy-3871R ATG18自噬相关蛋白18抗体
xy-3873R ATG4A自噬相关蛋白4A抗体
xy-3876R APOA4载脂蛋白A4抗体
xy-3874R ALOX1212脂氧合酶抗体
xy-3877R Angiotensin III血管紧张素III抗体
xy-4021R ADCY3腺苷酸环化酶3抗体