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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
IHC Antigen Retrieval Solution – Low pH (10X)
- 规格：
500ml
the heat induced epitope retrieval (HIER) step prior to immunohistochemistry
on formalin-fixed paraffin embedded tissue sections. The use of this buffer
in combination with heat (often by microwave, water bath, or pressure cooker)
has been shown to restore the antigenicity of proteins modified during the
formalin fixation of tissue. This buffer is supplied as a 10X stock solution.
Dilution of this buffer to 1X using reagent grade water results in a 10 mM
Sodium Citrate solution, pH 6.0.
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文献和实验抗原修复方法 Antigen Retrieval Methods
方法，玻片必须在开始IHC/ICC孵育之前冷却。HIER对时间、温度、缓冲液和pH值特别敏感，最佳方法必须通过实验确定。 热诱导抗原修复（HIER）方案 优化与局限性 抗原修复可能需要提高样本与载玻片或盖玻片的粘附性。此外，该技术通常对冰冻切片和酒精固定的组织过于苛刻。时间、温度和pH值也必须针对每种设备进行优化（例如，在92-95℃水浴中处理5-10分钟，或在120℃高压锅中处理1-5分钟）。为了优化抗原修复，必须使用一系列时间、温度和pH值组合进行初步研究。在使用任何抗原修复方法时，都应始终
) and unrelated proteins by formaldehyde groups. The great majority of monoclonal and polyclonal antibodies used for immunohistochemical (IHC) staining of formalin-fixed, paraffin-embedded (FFPE) tissues necessitate unmasking antigens for antigen retrieval
8.5. After dehydration with dimethylformamide, the specimens are embedded in LR-White resin. For antigen retrieval, ultrathin sections are heated in 20 mM Tris–HCl buffer (pH 9.0) for 1 h at 95�C. After immunogold labeling, the sections are treated with a mixture
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