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- 抗体英文名：
Mouse Cytokine Positive Control Cells
- 规格：
1ml
positive control cells when establishing an intracellular cytokine
staining protocol. Mouse splenocytes were stimulated with ConA (3 µg/mL) for
two days, followed by recombinant IL-2 (20 ng/mL) and IL-4 (20 ng/mL) for
three days. The cells were then restimulated with immobilized anti-mouse CD3 (10 µg/mL)
and soluble anti-mouse CD28 (2 µg/mL) in the presence of Brefeldin A for 5 hours.
These cells contain subpopulations that express IL-2, IL-3, IL-4, IL-10, GM-CSF,
and IFNγ. The cells have been fixed with formaldehyde and are provided in a
stabilizing buffer so that they may be stored at -80°C for up to six months
or at 2-8°C for up to one month.
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文献和实验Cytokine induced angiogenesis in chick embryos.
stage. This experiment investigates the embryonic stage of hematopoiesis, during which time blood vessels are created and circulation begins (Gilbert, 2003). The construction of blood cells occurs through two processes
Conditional Control of Gene Expression in the Mouse Retina
) cell, screening ES cell for the recombination events, injecting ES cells, and generating chimeric and null mice. It also discusses the current issues in the use of Cre/loxP -based gene-targeting approach.
Microglia from Progenitor Cells in Mouse Neopallium
This chapter outlines a procedure for initiation of mouse neopallium (cerebral cortex and its underlying white matter) cell cultures and their development into nearly pure microglia cultures. The cytokine CSF-1 is required for the development
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