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- 文献和实验
- 技术资料
- 抗体英文名:
IHC /ICC Blocking Buffer - Low Protein
- 规格:
50ml
and immunocytochemistry protocols which require blocking of non-specific binding sites.
This blocking buffer is recommended for tissues and antibodies with average levels of
background staining. This blocking buffer can be used both in the blocking step and
as a diluent for unconjugated antibodies, organic dye-conjugated, and eFluor® Nanocrystal
conjugated-antibodies during the antibody incubation step. This buffer is supplied as
a 1X stock solution containing sodium azide.
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文献和实验Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
Biotinylated anti‐prey antibody Pin cleaning solution (Genomic Solutions; see recipe ) Microarray blocking buffer (see recipe
with 1 X PBST three times, each for 10 minutes.The Trident Universal Protein Blocking Reagent (animal serum-free) (GTX30963) does not contain any animal serum, and can be used for all IHC and ICC kits, ELISAs, and WBs.以 ECL 系统检测信号1. Follow the instructions
Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions
, and analysis. A protocol is described for enhanced detection of low?affinity interactions by generating multivalent complexes using Fc?fusion bait proteins and protein A microbeads, along with a statistical method for hit scoring and identification
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