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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
ROOM TEMPERATURE
- 供应商:
西宝生物
- 库存:
500 g
- 英文名:
TRIS, ULTRA PURE
- 规格:
BIOCHEMICALS
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文献和实验E.Z.N.A. Cycle-Pure Kit Spin Protocol
. 9. Place HiBind® DNA column into a clean 1.5ml microcentrifuge tube. Add 30-50ul (depending on desired concentration of final product) of Elution Buffer (10mM Tris, pH8.5) or water directly onto the column matrix and centrifuge for 1 min at 13,000
培养细胞RNA提取--RNA Isolation Protocol-Cultured Cell Samples
Required Reagents: Chloroform Isopropanol 75% Ethanol (cold) Monophasic Isolation Reagent RNA Precipitation Solution 1.2M NaCl 0.8M Disodium Citrate DNase (RNase-free) Ultra-pure water Liquid Nitrogen Solutions
Magnetic stirrer Thermometer Reagents Alpha-Chymotrypsin (type II from Bovine pancreas) 0.1 g Calcium Chloride 0.1 g Ultra-pure water 100.0 ml 0.5% sodium hydroxide solution 0.5% hydrochloric acid solution Xylene Industrial methylated
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