- ¥3850
- Cloud-Clone Corp. (美国)
- 15.63-1000pg/mL
- SEB694Hu
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
研卉生物
- 检测范围：
15.63-1000pg/mL
- 检测方法：
酶联免疫吸附试验双抗夹心法
- 应用：
科学研究
- 标记物：
Interleukin 25 (IL25)
- 样本：
Serum, plasma and other biological fluids.
- 规格：
96T
ELISA Kit for Interleukin 25 (IL25)
产品编号 SEB694Hu 适用生物 Homo sapiens (Human，人)
品牌商 Cloud-Clone Corp. (美国)
检测范围 15.63-1000pg/mL 灵敏度 5.4pg/mL
样本类型 Serum, plasma and other biological fluids.
实验方法 双抗夹心法
| 产品编号 | 中文名称 | 英文名称 | 适用生物 | |
 SEB694Hu |
白介素25(IL25)检测试剂盒(酶联免疫吸附试验法) | ELISA Kit for Interleukin 25 (IL25) | Homo sapiens (Human) | |
| SEB694Mu |
白介素25(IL25)检测试剂盒(酶联免疫吸附试验法) | ELISA Kit for Interleukin 25 (IL25) | Mus musculus (Mouse) | |
| SEB694Ra |
白介素25(IL25)检测试剂盒(酶联免疫吸附试验法) | ELISA Kit for Interleukin 25 (IL25) | Rattus norvegicus (Rat) | |
| SCB694Hu |
白介素25(IL25)检测试剂盒(化学发光免疫分析法) | CLIA Kit for Interleukin 25 (IL25) | Homo sapiens (Human) | |
| SCB694Mu |
白介素25(IL25)检测试剂盒(化学发光免疫分析法) | CLIA Kit for Interleukin 25 (IL25) | Mus musculus (Mouse) | |
| SCB694Ra |
白介素25(IL25)检测试剂盒(化学发光免疫分析法) | CLIA Kit for Interleukin 25 (IL25) | Rattus norvegicus (Rat) |
| Organism species | Homo sapiens (Human) |
| Product No. | SEB694Hu |
| Sample type | Serum, plasma and other biological fluids. |
| Format | 96-well strip plate |
| Assay length | 4.5 hours |
| Detection range | 15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 5.4pg/mL. |
No significant cross-reactivity or interference between Interleukin 25 (IL25) and analogues was observed.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 98-105 | 102 |
| EDTA plasma(n=5) | 82-95 | 89 |
| heparin plasma(n=5) | 80-101 | 85 |
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 25 (IL25) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-97% | 79-103% | 87-94% | 85-97% |
| EDTA plasma(n=5) | 86-101% | 80-101% | 92-99% | 80-98% |
| heparin plasma(n=5) | 78-94% | 93-104% | 90-101% | 97-105% |
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人白介素-25 (IL-25)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 IL-25 单抗包被于酶标板上，标准品和样品中的 IL-25与单抗结合，加入生物素化的抗人 IL
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠白介素-25 (IL-25)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 IL-25 单抗包被于酶标板上，标准品和样品中的 IL-25与单抗结合，加入生物素化的抗小鼠
人可溶性白介素2R （sIL-2R/sCD25 ） 酶联免疫分析 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中可溶性白介素2R （sIL-2R/sCD25 ）的含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中人可溶性白介素 2R （ sIL-2R/sCD25 ）水平。用纯化的人可溶性白介素 2R （ sIL-2R/sCD25 ）抗体包被微孔板，制成固相抗体
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