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15.63-1000pg/mL
酶联免疫吸附试验双抗夹心法
科学研究
Interleukin 25 (IL25)
Serum, plasma and other biological fluids.
96T
产品编号 | 中文名称 | 英文名称 | 适用生物 | |
SEB694Hu | 白介素25(IL25)检测试剂盒(酶联免疫吸附试验法) | ELISA Kit for Interleukin 25 (IL25) | Homo sapiens (Human) | |
SEB694Mu | 白介素25(IL25)检测试剂盒(酶联免疫吸附试验法) | ELISA Kit for Interleukin 25 (IL25) | Mus musculus (Mouse) | |
SEB694Ra | 白介素25(IL25)检测试剂盒(酶联免疫吸附试验法) | ELISA Kit for Interleukin 25 (IL25) | Rattus norvegicus (Rat) | |
SCB694Hu | 白介素25(IL25)检测试剂盒(化学发光免疫分析法) | CLIA Kit for Interleukin 25 (IL25) | Homo sapiens (Human) | |
SCB694Mu | 白介素25(IL25)检测试剂盒(化学发光免疫分析法) | CLIA Kit for Interleukin 25 (IL25) | Mus musculus (Mouse) | |
SCB694Ra | 白介素25(IL25)检测试剂盒(化学发光免疫分析法) | CLIA Kit for Interleukin 25 (IL25) | Rattus norvegicus (Rat) |
Organism species | Homo sapiens (Human) |
Product No. | SEB694Hu |
Sample type | Serum, plasma and other biological fluids. |
Format | 96-well strip plate |
Assay length | 4.5 hours |
Detection range | 15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL |
Sensitivity | The minimum detectable dose of this kit is typically less than 5.4pg/mL. |
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 98-105 | 102 |
EDTA plasma(n=5) | 82-95 | 89 |
heparin plasma(n=5) | 80-101 | 85 |
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 90-97% | 79-103% | 87-94% | 85-97% |
EDTA plasma(n=5) | 86-101% | 80-101% | 92-99% | 80-98% |
heparin plasma(n=5) | 78-94% | 93-104% | 90-101% | 97-105% |
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
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