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西宝生物
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文献和实验Midiprep preparation of Plasmid DNA
实验概要 The PureLink™ HiPure Plasmid DNA Midiprep Kit allows purification of 100–350 μg of high-quality plasmid DNA from 15–25 mL overnight E. coli cultures in ~2 hours when cloning high copy number plasmids
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
ethanol (room temperature, 96-100%). Gently mix by inverting tube 6-7 times and incubate at room temperature for1-2 minutes. Plasmid DNA Purification with the HiBind® DNA Mini Column II: 8. Equilibrate the HiBind® DNA column: Add 100ul
E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
for removing ethanol from the column. 15. Place column into a new clean 1.5 ml micro-centrifuge tube. Add 30-50 u (depending on desired concentration of final product) Endotoxin-Free Elution Buffer (or water) directly onto the column matrix and centrifuge
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