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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
西宝生物
- 库存:
大量
- 规格:
10KU
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文献和实验. 6. 10× Tango buffer (Thermo Scientifi c/Fermentas). 7. DTT (DL-Dithiothreitol; Cleland’s reagent). 8. 10 mM Adenosine 5′-triphosphate. 9. Ultrapure water (RNAse/DNAse-free). 10. Tris-EDTA (TE) buffer. 11. E. coli competent cells. Strains
Sample preparation (preparative gels)
) and a trace of bromophenol blue. After centrifugation at 10000 g for 5 minutes, the whole final E. coli diluted sample (5 mg) was used for in-gel sample rehydration. YEAST (Saccharomyces cerevisiae): Cells were washed twice with PGSK (NH2PO4-H2O 0.52
Sample preparation (analytical gels)
, the whole final diluted E. coli sample was loaded onto the IPG gel strip. YEAST (Saccharomyces cerevisiae): Cells were washed twice with PGSK (NH2PO4-H2O 0.52 g/l, Na2HPO4-2H2O 8.8 g/l, NaCl 2.83 g/l, KCl 0.372 g/l and glucose 11 g/l), centrifuged at 3000 rpm
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