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- 供应商:
西宝生物
- 库存:
大量
- 规格:
1CS
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文献和实验E.Z.N.A. Mag-Bind Blood DNA Protocol (1-100 ul Blood)
2. 500 ul Nuclease-free microplate 3. 1.2mL deep well plate (EV1772) 4. 8-strip caps for 1.2mL deep well plate (EV1774) 5. Water bath, incubator or heating block preset at 65° C 6. Magnetic separation device for microcentrifuge
increase with increasing fixation time. Earlier protocols used short fixation times (e.g. 10 minutes). For MCD1-6HA or MCD1-18MYC, 2 h. fixation worked well. Transfer cells to 50 mL or 250 mL conical tubes and spin down in clinical centrifuge
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
. Centrifuge for 5 min and wash pellet with 70 % EtOH 15. Dry pellet and resuspend the two tubes in a total of 50 l TE-buffer+RNase, use 2ml for a PCR, freeze the rest. MPS1 for 50 ml Stock 50 mM glucose 1M 2,5 ml 10 mM EDTA 0,5mM 1 ml
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