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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-15℃避光防潮
- 保质期:
12个月
- 库存:
1
- 供应商:
西安百萤生物科技有限公司
1mM Fluorescein-dUTP溶液
| 货号 | 17022 | 存储条件 | f/l |
| 规格 | 25 nmoles | ||
| Ex (nm) | 497 | Em (nm) | 516 |
| 分子量 | 1060.62 | 溶剂 | Water |
简要概述
产品基本信息
货号:17022
产品名称:1mM 荧光素-dUTP溶液
规格:25nmolse
储存条件:-80℃避光干燥
保质期:12个月
产品物理化学光谱特性
分子量:1060.62
外观:液体
溶剂:10mM Tris-HCl + 1mM EDTA (pH=7.5)
激发波长(nm):492
发射波长(nm):515
产品介绍
染料修饰的脱氧尿苷5'-三磷酸(例如氨基烯丙基-dUTP)可用于通过常规酶掺入方法如逆转录,切口平移,随机引物标记或PCR产生含染料的DNA。该酶荧光标记方法广泛用于FISH探针和基于微阵列的实验。该荧光素-dUTP缀合物可用作具有Spectrum Green™过滤器组的绿色荧光颜色(Spectrum Green ™是Vysis的商标)。
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文献和实验Random Prime Labeling of DNA Probes with Fluorescein-11-dUTP
The selection of an appropriate labeling reagent for a particular experiment, for the most part, depends on the sensitivity and resolution required. For maximum sensitivity in filter hybridizations radioactive labels, such as phosphorous 32
3′-End Labeling of Oligonucleotides with Fluorescein-11-dUTP and Enhanced Chemiluminescent Detection
In this reaction, the enzyme terminal transferase is used to introduce a short tail of fluorescein nucleotides to the 3′-end of an oligonucleotide (1 ) followed by horseradish peroxidase-catalyzed enhanced chemiluminescent detection
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
microscope. Utilizing the incorporation of fluorescein-labeled nucleotides, the use of fluorescence microscopy is also possible. B) TUNEL in situ procedure 2. PROTOCOLS A.2.1 Materials Bouin's fixative (A1), alcohols
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