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文献和实验Microarrays for Universal Detection and Identification of Phytoplasmas
Detection and identification of phytoplasmas is a laborious process often involving nested PCR followed by restriction enzyme analysis and fine-resolution gel electrophoresis. To improve throughput, other methods are needed. Microarray
FISH Detection on DAPIBanded Chromosomes
, however, to precisely position the hybridization signals with respect to the conventional chromosome bands. Various approaches have been introduced, including G banding before or after FISH detection (2 , 3 ), cohybridization with Alu or L 1 probes to generate R or G
Application of FISH to Detect DNA Damage: DNA Breakage Detection-FISH (DBD-FISH)
area to be analyzed. In summary, DBD-FISH integrates the microgel techniques habitually employed in the “nuclear halo” (5 ,6 ) or “comet tail” tests (7 ,8 ), the alkaline unwinding assays for the detection of DNA breaks currently used
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