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文献和实验: A Software Package for the Design of Primers Dedicated to Whole-Genome Scanning by LR-PCR Nouri Ben Zakour; Yves Le Loir pp. 349 - 368 19. Designing PCR Primer for DNA Methylation Mapping Long-Cheng Li pp. 371 - 384 20. BiSearch: ePCR Tool
PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
of this is nested primer PCR: PCR amplification is performed with one set of primers, then some product is taken - with or without removal of reagents - for re-amplification with an internally-situated, “nested” set of primers. This process adds another level
Real-Time Primer Design for DNA Chips
an optimization of a specified primer set in real-time.1 IntroductionBoth, the amplification of DNA sequences using polymerase chain reaction (PCR) and the massive parallel analysis of genes in biological cells using DNA chips (or DNA arrays) have a great impact
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