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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
) (see Step 39) The kit includes end-repair enzyme mix, end-repair 10X buffer, dNTP solution, and ATP. Dynabeads Protein A (Invitrogen) Ethanol (70% and 100%) Glycerol Glycogen, 20 mg/mL Klenow fragment exo- (optional; see Step
. 45-46 for instructions to shear DNA.**Important: Before shearing, DNA must be in solution, or the Hydroshear will clog.II. DNA PrecipitationAfter shearing, precipitate DNA with 1/10 volume 3M NaOAc, and 2 volumes ethanol. Spin and wash with 70
in a screw cap eppendorf tube for 5 mins. Leave on ice for >5 mins 11. Wash solution II (2 L, use 500 mL per wash for each container): Mix 100 mL of 20X SSC (1X), 10 mL of 20% SDS (0.1%), and dH2 O to 2 L
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