406 ANALOG WO/PUMP =E241833

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

) connected to a vacuum pump (KNF) Basic Protocol 3: Synthesis, Purification, and Characterization of the Mono‐6‐Thioated Analog of 2′‐O‐Propargylated Cyclic‐DI

Identification of a Mutant Kinase/ATP Analog Pair

  Identification of a Mutant Kinase/ATP Analog Pair   Scott T. Eblen, N. Vinay Kumar, and Michael J. Weber Department of Microbiology and Cancer Center, University of Virginia Health

Use of a Fluorescent ATP Analog to Probe the Allosteric Conformational Change in the Active Site of the Protein Kinase PDK1

activity in vitro, e.g., small compounds can activate the phosphoinositide-dependent protein kinase 1 (PDK1). Compounds binding to an allosteric site on a protein kinase may produce conformational changes at the ATP-binding site within the active site