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文献和实验of 7 x 2 x 0.5" and bottom dimensions of 7 x 2 x 0.25". 2) Recess the top plate in the center portion to 0.125" deep, leaving 0.950" per end for 1.375" wide x 0.3125" deep fluid reservoir. 3) Drill 1/16" wide x 0.625" long
Transplantation of Pancreatic Islets Into the Kidney Capsule of Diabetic Mice
Preparation of Islets for Transplant (Tx) Under an inverted microscope, hand-pick islets using a P200 pipetman and straight pipet tip from the cultured islets in a 100mm plate. Count islets 100 at a time and transfer
) that are subsequently polished (blunted). Short adaptors are then ligated onto the ends of the fragments. These adaptors provide priming sequences for both amplification and sequencing of the sample-library fragments. Adaptor B contains a 5'-biotin tag that enables
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