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文献和实验I buffer (Tris pH8.4, 20 mM MgCl2 , 500 mM KCl) 5 µL DNAse I (RNAse free) q.s. 100 µL with DEPC H2 O Incubate at room temp. x10 min. Immediately proceed to next step. RNeasy Mini Prep Procedure
Streptomyces:Protocols/Mini-Maxi Prep
Small Scale Plasmid Isolation (Mini / Maxi Prep) Description A mini prep / maxi prep is used to isolate plasmid or cosmid DNA from bacteria, normally E.coli. An overnight culture of cells is lysed and the majority of the cells
【转帖】2-D Clean-Up Kit 双向凝胶电泳--蛋白纯化
in the 1st dimension. The 2D Clean-Up Kit from GE Healthcare (formerly Amersham Biosciences) is the most effective method of getting rid of such impurities and thus, sample preparation with this kit results in proper isoelectric focusing and minimal streaking
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