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文献和实验Digital Gene Expression by Tag Sequencing on the Illumina Genome Analyzer
a protocol for performing digital gene expression profiling on the Illumina Genome Analyzer sequencing platform. Tag sequencing (Tag?seq) is an implementation of the LongSAGE protocol on the Illumina sequencing platform that increases utility while reducing
Native Chromatin Preparation and Illumina/Solexa Library Construction
, including the Genome Analyzer (Solexa/Illumina), 454 (Roche), and ABI-SOLiD (Applied Biosystems). This protocol describes sample preparation for sequencing of chromatin-immunoprecipitated DNA (ChIP-Seq) to analyze histone modification patterns using
Summary: Illumina's Solexa Sequencing Technology
by Bridge AmplificationIn contrast to the 454 and ABI methods which use a bead-based emulsion PCR to generate "polonies", Illumina utilizes a unique "bridged" amplification reaction that occurs on the surface of the flow cell.The flow cell surface is coated
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