KIT, CEQ -47 SEQUENCING PRIMER

Sequencing Protocol

content of Bisulfite converted DNA is not as drastically altered as is the individual base composition.Template Quality and QuantityReference: BigDye Terminator v1.1 Cycle Sequencing Kit Protocol, Applied Biosystems 2002 (Part# 4337036A), BigDye Terminator

SOLiD Sequencing

Applied Biosystems has just launched their instrument, which supports their version of high-throughput sequencing chemistry, termed “SOLiD™” (little “i”, please). Acquired from Agencourt Personal Genomics in late 2006, SOLiD is a unique parallel

Sequencing Double-Stranded DNA Using the Sequenase Version 2.0 Kit

. 3. For each sample to be sequenced, put the following in a 1.5 ml Eppendorf tube: DNA (prepared as discussed) 7 ul Sequencing primer 1 ul Reaction Buffer 2 ul 4. Heat to 65 degrees for 2 minutes, then remove heating block