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文献和实验Maintenance of Probes in bacteria including Escherichia coli
agar. Use airtight, autoclaved vials containing 2/3 full (appr. 1 ml) of medium. Inoculate each vial with a single colony. Leave the cap of the vial sightly loose and incubate overnight at 37 C.
Maintenance of Probes in bacteria including Escherichia coli
colony. Leave the cap of the vial sightly loose and incubate overnight at 37 C. Seal the vial tightly and stored at room temperature in a dark place. To revive
Joe's mRNA prep (Audrey Gasch, Pat Brown [modified by S.M. Hettenbach]) Oligo-dT cellulose prep Dump Ambion vial contents into 50 ml c/f tube Add 10 ml 1x NETS to vial, cap, rinse vial, dump 10 ml into c/f tube
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