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文献和实验Cells: We grow our CHOs with MEM[[alpha]] (without nucleosides) + 10% Bovine Calf Serum and penn/strep/glutamine. For a prep it is best
CHO Centrosome Prep: Arshad Desai 4/94 Cells: We grow our CHOs with MEM[[alpha]] (without nucleosides
column (clamped to a support in the cold room) to fill it to the top. Cover the column with parafilm, and allow the resin to settle for several hours. Fill the column adjuster plunger with column buffer, and VERY slowly insert
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