SPACER,INS

Ins and Outs of Mycobacterial Plasmids

The importance of plasmids for molecular research cannot be underestimated. These double-stranded DNA units that replicate independently of the chromosomal DNA are as valuable to bacterial geneticists as a carpenter’s hammer. Fortunately

Internal Transcribed Spacer (ITS)-PCR Identification of MRSA

Polymerase chain reaction (PCR) analysis of the 16S-23S rRNA gene internal transcribed spacer (ITS) followed by microchip gel electrophoresis was useful for identification of staphylococci and for strain delineation of Staphylococcus aureus

Analysis of Signal Transduction: Formation of cAMP, cGMP, and Ins(1,4,5)P3 In Vivo and In Vitro

of the second messengers cAMP, cGMP, and Ins(1,4,5)P3 in cells will provide information on the entire pathway; here, details on isotope-dilution assays, through which the concentrations of these second messengers can be determined, are offered. Subsequently