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文献和实验galactosidase assay for cells transfected with pSV- -Galactosidase Vector
absorbance at 420 nm. All components for b-gal assay are on top shelf of -20℃. All components for Luciferase assay are in Common Rack B. For info about this kit and map of pSV-b-gal vector, look in the cardboard box of kit manuals by Fan’s office.
of TBS-mod buffer, vortexed and centrifuged for 30 seconds at 6,000 RPM(comment 4); ;Gently mix (rotating plate) the cell lysate/ChIP pulldown with the antibody-M2 beads for 2-3 hours or overnight at 4°C;Capture the Flag-antibody-M2 immunoprecipitated
DMS Chemical Footprinting of RNA
Buffer kit) 3.1 mL H2O *Dilute RNA to 1 uM in 1x CE buffer Conditions Condition 1: 10 uL RNA (1 uM concentration) 2.5 uL 10x CE buffer 12.5 uL H2O Condition 2: 10 uL RNA
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