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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
15. DPBS Without Calcium or Magnesium 16. TRIzol® LS Reagent 17. SuperScript® VILO™ cDNA Synthesis Kit 18. AccuPrime™ SuperMix I 19. Mouse Anti-Tra1-60 Antibody 20. Mouse Anti-Tra1-81 Antibody 21. Mouse Anti-SSEA
E.Z.N.A. Cycle-Pure Kit Spin Protocol
products 3. Vortex thoroughly to mix. Briefly spin the tube to collect any drops from the inside of the lid. 4. Place a HiBind® DNA column in a provided 2ml collection tube. Add 100ul Equilibration Buffer to the column. Incubate at room
Plasmid Mini Purification Protocol
after addition of Buffer P3.Mix by inverting the tube 4-6 times. 4. Centrifuge at maximum speed in a microfuge for 10 min (or at 21,000 RPM in the F2402 rotor in the Beckman Avanti 30 centrifuge for 10 min).Remove supernatant promptly.Do step
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