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LAB EQUIPMENT,BOTTLE,3000ML
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ChIP Protocol-Myerslab-112607[1].pdf
Marcantonio Lab Protocol Manual
cold room. 3) Aliquot culture into 8 pre-chilled sterile 50 ml polypropylene tubes. Let stand on ice for 10 minutes. 4) Centrifuge cells for 7 minutes at 3000 rpm at 4 degrees. 5) Pour off the supernatant and gently resuspend each pellet in 10 ml
: Caution: Wear gloves and avoid getting DEPC on your skin. RNase free water (make several liters to make up the running buffer. Having some 100 ml bottles is also good.) To ddH 2 O, add DEPC to 0.1%
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LAB EQUIPMENT,BOTTLE,3000ML
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