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文献和实验In Vivo cII, gpt, and Spi Gene Mutation Assays in Transgenic Mice and Rats
), and gpt -delta (rats or mice). The Big Blue� and Muta™ mouse models use the cII gene as a reporter of mutation whereas gpt -delta rodents use the gpt gene and the red /gam genes (Spi− selection) as mutation reporter genes. Here we describe methodology
Antibiotic concentrations for E. coli selection
) to 100 mL of LB. Reagent List: Antibiotics Unless otherwise indicated, the antibiotic powder can be dissolved in H2 0. Addgene recommends making 1000X stock solutions and storing aliquots at -20o C. Reagent
Proximity-Guided (ProxiMol) Antibody Selection
Cell surfaces provide a rich source of potential antigen (Ag) targets for therapeutic and research reagent antibodies (Abs). However, in some circumstances, access to these targets may be difficult since it is technically challenging
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