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- Bio-Rad伯乐生命科学
- 美国
- 1708898
- 2025年07月09日
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文献和实验Procedure 1. Homogenize cells (10 million) or tissue (50-100 mg) in 1 mL TRIzol Reagent (e.g. scrape and pass through 30G needle, dounce homogenize and pass through needle, or use a homogenizer) and transfer to a 1.5 mL tube.
at -20C in 1ml aliquots Alternate 5x solution: 5g/42ml PMSF: 0.3M stock solution in DMSO; use 1:1000 Kanamycin: 0.25g/10ml, use 1:1000 only for cells with [pREP-4] plasmid! ORDERING INFO Sigma: Glutathione beads, cat
Preparation of BAC DNA with by Alkaline Prep Method
CLONE ISOLATION PREP - PROTOCOL Before you begin isolating DNA from the cultures: --GENTLENESS is key through step 10! --Try to do prep steps 1-8 at the side of the centrifuge --Make Solution III and place in fridge or on ice morning
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