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- merck-millipore
- 05-719NA-KL
- 2025年07月12日
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北京盛科博源生物科技有限公司
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文献和实验background when used for immunocytochemistry, although we have found that this can be eliminated by both minimizing the spheroplasting process and preadsorbing primary and secondary antibodies in multiple incubations against fixed yeast cells suspended in PBS
Anti-HA Immunocytology The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots
1. Treat cells. For PC12 or NIH 3T3 cells a moderately confluent 60 mm plate is used. 2. Aspirate media. Rinse cells with PBS, aspirate.3. Lyse cells in 200 uL of SDS sample buffer (100 ℃). Immediately scrape the cells off the plate with a rubber
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