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文献和实验sequences. In addition, heterochromatin cannot be analyzed effectively on polytene chromosomes because it is underreplicated and included in the chromocenter. However, by applying high-resolution banding techniques (e.g., quinacrine, Hoechst, and N-banding
又叫荧光分带法。用氮芥喹吖因(quinacrine)荧光染料染色, 在紫外光激发下,显现明暗不同的带区,可在荧光显微镜下观察。一般富含AT碱基的DNA区段表现为亮带, 富含GC碱基的区段表现为暗带。此法的优点是分类简便, 可显示独特的带型。缺点是标本易褪色,不能做成永久性标本片。
FISH Detection on DAPIBanded Chromosomes
bands (4 ), use of a combination of various fluorescent dyes, such as quinacrine, Hoechst 33258, or DAPI, with FISH detecting reagents (5 , 6 ), and triple staining with chromomycin, distamycin A, and DAPI (7 , 8 ). Unfortunately, these procedures
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