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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
一年以上
- 英文名:
SYBR® Select Master Mix, 1-Pack (1 x 5 mL) (Applied Biosystems®)
- 库存:
现货
- 供应商:
武汉科昊佳生物科技有限公司
- CAS号:
******
- 保存条件:
4度冷藏
- 规格:
500 reactions
| 品名 | 货号 | 品牌 | 规格 | 报价 |
| SYBR® Select Master Mix, 1-Pack (1 x 5 mL) (Applied Biosystems®) |
4472908 | Life-ABI美国应用生物Applied Biosystems | 500 reactions | ¥2,400 |
描述
SYBR® Select Master Mix is a superior and cost-effective solution for your real-time PCR applications.• Specific—minimize primer-dimer and non-specific amplification
• Reproducible and sensitive—consistent amplification across a wide dynamic range
• Bright—contains SYBR® GreenER™ dye for maximum brightness
• Carry-over contamination control—contains heat-labile UDG
• Broad instrument compatibility
Formulated for Maximum Specificity
SYBR® Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq® DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.
Obtain Reproducible Results Across a Wide Dynamic Range
SYBR® Select Master Mix is specially formulated to provide robust results from 100 ng to 0.1 pg cDNA per reaction and can reliably detect a single copy of genomic DNA.
Contains SYBR® GreenER™ Dye and Heat-labile UDG
• SYBR® GreenER™ dye is less inhibitory to PCR than SYBR® Green I dye resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control
Instrument Compatibility
SYBR® Select Master Mix is used in standard cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments. It is also compatible with the Bio-Rad IQ™5, Roche LightCycler® LC480, and Stratagene® MX3005P® systems.
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
内容和储存
The 2x mix contains SYBR® GreenER™ dye, AmpliTaq® DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 5 mL tube, sufficient for 500, 20 µL reactions (10 µL Master Mix per reaction) . Store at 2 - 8 °C.详细说明
常用规格
| Detection Method: | SYBR |
| Polymerase: | AmpliTaq® DNA Polymerase |
| Purity or Quality Grade: | UP (Ultra Pure) |
| Platform: | StepOne™, Standard Mode, ViiA™ 7 System, 7900HT System, Standard Mode, StepOnePlus™, Standard Mode, 7500 System, QuantStudio™ 12K Flex |
| Reaction Speed: | Standard |
| Sample Type (General): | DNA |
| Sample Type (Specific): | cDNA, Genomic DNA |
| Primer-Probe Compatibility: | Unlabeled Oligos |
| Form: | Liquid |
| Format: | Vial(s) |
| Hot Start: | Built-In Hot Start |
| PCR Method: | qPCR |
| Label or Dye: | SYBR® GreenER™ |
| Product Size: | 500 reactions |
| cGMP Compliance: | non-cGMP Compliant |
| Shipping Condition: | Wet Ice |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
| Passive Reference Dye: | ROX (Pre-mixed) |
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文献和实验1992 年,Higuchi 在一次报告中提出了实时荧光定量 PCR 技术。通过检测溴化乙锭的含量实时监控整个 PCR 反应的进程。之后经过不断的研究,通过对 PCR 反应的数学函数关系、相应的算法以及对标准品的运用,就可以对待测样品中的目标基因进行准确定量。 1996 年 1996 年 ABI 公司推出世界上第一台定量 PCR 仪 7700 型。设备由荧光定量系统和计算机组成,通过荧光染料或荧光探针,对 PCR 产物进行标记跟踪,结合相应的软件对结果进行分析,可以实现计算待测样品
了长足的发展,比如BioTeke公司的荧光定量系列产品就以其优良的品质和亲民的价格越来越受到国内用户的青睐,目前还可以申请试用装。为了证明其荧光定量系列产品的品质,BioTeke公司特邀请了山东大学细胞发育遗传学实验室进行了BioTeke的SYBR GREEN I实时荧光定量产品和T公司的实时荧光定量试剂的比对实验。具体实验如下:实验材料小鼠肝脏组织山东大学实验室提供样品cDNA实验方法RNA提取:Bioteke RNApure高纯总RNA提取试剂盒,操作步骤请参考说明书。目的基因:小鼠管家基因β–
的 microRNA; ● 超宽的线性范围,可跨越 7 个数量级。 实时定量 PCR 的系统构成及工作原理 荧光定量检测系统由实时荧光定量 PCR 仪,实时荧光定量试剂,通用电脑,自动分析软件等构成。设备由荧光定量系统和计算机组成,用来监测循环过程的荧光。 与实时设备相连的计算机收集荧光数据。数据通过开发的实时分析软件以图表的形式显示。原始数据被绘制成荧光强度相对于循环数的图表。原始数据收集后可以开始分析。实时设备的软件能使收集到的数据进行正常化处理来弥补背景荧光的差异。正常化后可以设定域值水平
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