igure 1: Melt curve analysis for 3 targets run on QuantStudio® 7 instrument for 5 different master mixes. Manufacturer’s recommended cycling conditions were used for each mix over a 6 log dilution series of human universal reference cDNA. Competitor B: BioRad (SsoAdvanced™Universal SYBR® Green Supermix); Competitor R: Roche (LightCycler® 480 SYBR® Green I Master); Competitor QB: Quanta Bioscenices (PerfeCTA® SYBR® Green SuperMix ); Competitor Q: Qiagen (QuantiTect SYBR® Green PCR Kit).
Figure 2: Quadruplicate reactions were run for 24 assays at 300 nM over two lots of PowerUp™ SYBR® Master Mix on the 7900 instrument. Standard deviation of the Ct is plotted vs the Average Ct. Average Cts >34 were excluded from this analysis.