- 询价
- ABI
- USA
- 4472908
- 2026年04月24日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保质期：
1年以上
- 英文名：
SYBR Select Master Mix, 1-Pack (1 x 5 mL)
- 库存：
现货
- 供应商：
科昊佳生物
- 保存条件：
4°C
- 规格：
500 reactions
| 品名 | 货号 | 品牌 | 规格 | 报价 |
| SYBR® Select Master Mix, 1-Pack (1 x 5 mL) (Applied Biosystems®) |
4472908 | Life-ABI美国应用生物Applied Biosystems | 500 reactions | ¥2,199 |
SYBR® Select Master Mix is a superior and cost-effective solution for your real-time PCR applications.
• Specific—minimize primer-dimer and non-specific amplification
• Reproducible and sensitive—consistent amplification across a wide dynamic range
• Bright—contains SYBR® GreenER™ dye for maximum brightness
• Carry-over contamination control—contains heat-labile UDG
• Broad instrument compatibility
Formulated for Maximum Specificity
SYBR® Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq® DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.
Obtain Reproducible Results Across a Wide Dynamic Range
SYBR® Select Master Mix is specially formulated to provide robust results from 100 ng to 0.1 pg cDNA per reaction and can reliably detect a single copy of genomic DNA.
Contains SYBR® GreenER™ Dye and Heat-labile UDG
• SYBR® GreenER™ dye is less inhibitory to PCR than SYBR® Green I dye resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control
Instrument Compatibility
SYBR® Select Master Mix is used in standard cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments. It is also compatible with the Bio-Rad IQ™5, Roche LightCycler® LC480, and Stratagene® MX3005P® systems.
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
内容和储存
The 2x mix contains SYBR® GreenER™ dye, AmpliTaq® DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 5 mL tube, sufficient for 500, 20 µL reactions (10 µL Master Mix per reaction) . Store at 2 - 8 °C.详细说明
常用规格
| Detection Method: | SYBR |
| Polymerase: | AmpliTaq® DNA Polymerase |
| Purity or Quality Grade: | UP (Ultra Pure) |
| Platform: | StepOne™, Standard Mode, ViiA™ 7 System, 7900HT System, Standard Mode, StepOnePlus™, Standard Mode, 7500 System, QuantStudio™ 12K Flex |
| Reaction Speed: | Standard |
| Sample Type (General): | DNA |
| Sample Type (Specific): | cDNA, Genomic DNA |
| Primer-Probe Compatibility: | Unlabeled Oligos |
| Form: | Liquid |
| Format: | Vial(s) |
| Hot Start: | Built-In Hot Start |
| PCR Method: | qPCR |
| Label or Dye: | SYBR® GreenER™ |
| Product Size: | 500 reactions |
| cGMP Compliance: | non-cGMP Compliant |
| Shipping Condition: | Wet Ice |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
| Passive Reference Dye: | ROX (Pre-mixed)
|
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验了我的扩增曲线呢？ 其实，这种现象并不是偶发的，有很多原因可能导致，并且有个专属名称「Hook Effect」，翻译过来叫「鱼钩效应」。「鱼钩效应」指在荧光定量 PCR 过程中，DNA 扩增到指数增长期后出现的一种荧光信号不能保持稳定（或者上升）而出现下降的现象 [1]。 导致扩增曲线出现这一现象的因素比较复杂，这里我们分别从嵌入性染料法与探针法两种定量方式的角度为大家解释： 嵌入性染料法： 嵌入性染料法（例如 SYBR Green I）产生「鱼钩效应」主要是由于模板序列混杂，在 PCR
了长足的发展，比如BioTeke公司的荧光定量系列产品就以其优良的品质和亲民的价格越来越受到国内用户的青睐，目前还可以申请试用装。为了证明其荧光定量系列产品的品质，BioTeke公司特邀请了山东大学细胞发育遗传学实验室进行了BioTeke的SYBR GREEN I实时荧光定量产品和T公司的实时荧光定量试剂的比对实验。具体实验如下：实验材料小鼠肝脏组织山东大学实验室提供样品cDNA实验方法RNA提取：Bioteke RNApure高纯总RNA提取试剂盒，操作步骤请参考说明书。目的基因：小鼠管家基因β–
表 Part 3：Plate Setup 设置布板信息 完成了 Experiment Properties 的设置后就进入了 Plate Setup 布板页面，此时我们需要分别进行 Define Targets and Samples 步骤去定义我们待检测 Target 的荧光标记策略和样本信息，以及 Assign Targets and Samples 步骤来完成对样品及待检测目标具体孔位分布信息的注释。 其中定义 Target 时，如果我们选择的是染料法，那么 Reporter 下选择 SYBR 而 Quencher
技术资料暂无技术资料 索取技术资料
