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- 详细信息
- 文献和实验
- 技术资料
- 宿主:
不限
- 供应商:
西宝生物
- 库存:
大量
- 抗原来源:
不限
- 是否单克隆:
0
- 保存条件:
Store at -20° C,
- 规格:
10 µM
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文献和实验., Jarvis, R., Pallotta, V., Byrom, M., and Ford, L. (2002) RNA interference in mammalian cell culture: design, execution, and analysis of the siRNA effect. Ambion TechNotes 9(1): 3-5. 4. Sui, G., Soohoo, C., Affar, E.B., Gay, F., Shi, Y., Forrester, W.C
Using siRNA for gene silencing is a rapidly evolving tool in molecular biology. There are several methods for preparing siRNA, such as chemical synthesis, in vitro transcription, siRNA expression vectors, and PCR expression cassettes
the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
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