- ￥2780
- peprotech
- 500-P07G-50UG
- 2025年07月13日
- WB, IHC-P, ELISA
- 兔
- 人
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- 详细信息
- 文献和实验
- 技术资料
- 目录编号：
500-P07G-50UG
- 克隆性：
多克隆
- 适应物种：
人
- 宿主：
兔
- 应用范围：
WB, IHC-P, ELISA
- 靶点：
抗人白介素17A抗体
- 抗体英文名：
IL-17A Polyclonal Antibody
- 抗体名：
抗人白介素17A抗体
- 规格：
50ug
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
0.1-0.2 µg/mL | 查看2篇已发表文章 |
免疫组化 (IHC) |
- | 查看1篇已发表文章 |
酶联免疫吸附实验 (ELISA) |
0.5-2.0 µg/mL | - |
| 产品规格 | |
|---|---|
种属反应 |
Human |
已发表种属 |
Human |
宿主/亚型 |
Goat |
分类 |
Polyclonal |
类型 |
Antibody |
Source: Polyclonal Rabbit
Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-17A. Anti-Human IL-17A specific antibody was purified by affinity chromatography employing immobilized hIL-17 matrix.
Immunogen: E.coli derived Recombinant Human IL-17A (PeproTech catalog# 200-17)
Sandwich ELISA: To detect hIL-17A by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-17A (500-P07Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17A.
Western Blot: To detect hIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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文献和实验American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
The importance of testing anti-IL-17 antibodies from different suppliers.
"500-P07G was used in Immunohistochemistry to highlight the importance of testing anti-IL-17 antibodies from different suppliers."
Molecular therapy : the journal of the American Society of Gene Therapy
Neither IL-17A mRNA nor IL-17A protein are detectable in Langerhans cell histiocytosis lesions.
"500-P07G was used in Western Blotting to investigate the etiology of Langerhans cell histiocytosis (LCH) to demonstrate a failure to identify any cells in LCH lesions with interleukin-17 (IL-17A) gene expression."
Journal of immunology (Baltimore, Md. : 1950)
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人白介素-17A (IL- 17A )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 IL-17A 单抗包被于酶标板上，标准品和样品中的 IL-17A与单抗结合，加入生物
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠白介素-17A (IL-17A)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 IL-17A 单抗包被于酶标板上，标准品和样品中的 IL-17A与单抗结合，加入生物
Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇：make an Antibody against a Peptides 下一篇：Immunization Protocol for Monoclonal Antibody Production
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