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阿拉丁试剂,充足现货,稳定批间差abinScience 体内级抗体,年度锁价,低至 1200五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
6 months from date of receipt.
- 英文名:
Human Fab Of Normal IgG
- 库存:
99
- 供应商:
Biorbyt
- 规格:
10 mg
描述: Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent in a variety of immunoassays including immunodiffusion, immunoelectrophoresis, ELISA, Western blotting, dot-immunobinding assays (DIBA), haemagglutination, and cell-binding assays.
应用: DOT, ELISA, WB
RRID: AB_10749333
研究领域: Epigenetics & Chromatin
保存条件: After arrival store at +4°C. Prolonged storage may be at -20°C. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Reconstituted solution may be stored frozen. Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.
备注: For research use only.
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文献和实验Production of Recombinant Human IgG Antibodies in the Baculovirus Expression System
Despite antibody fragments like scFv or Fab are sufficient for various tasks, complete recombinant IgG is sometimes needed. Production of IgG molecules is not generally achievable in E. coli . Insect cells are able to produce IgG and the use
【摘要】目的探讨嵌合抗CD20抗体Fab’片段的抗肿瘤机制。方法应用MTT法检测Fab’片段对Raji细胞生长的抑制作用,用Annexin V-FITC和PI测定Fab’片段对Raji细胞凋亡的诱导作用,用RT-PCR和Western blot检测Raji细胞中bcl-2表达的变化。结果嵌合抗CD20Fab’片段对Raji细胞具有明显的抑制作用IC50值为24.2 µg/ml,并呈剂量依赖性;嵌合抗CD20Fab’片段能诱导Raji细胞的凋亡,并降低Raji细胞中bcl-2基因及其蛋白的表达
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