IRE1 Rabbit Polyclonal Antibody 抗体,orb157705,Biorbyt产品图

IRE1 Rabbit Polyclonal Antibod

y 抗体,orb157705,Biorbyt
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  • ¥3159 - 5850
  • Biorbyt已认证
  • orb157705
  • 英国
  • 2026年07月22日
  • IF, IHC-Fr, IHC-P
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 抗体名

      IRE1 Rabbit Polyclonal Antibody 抗体

    • 抗体英文名

      IRE1 Rabbit Polyclonal Antibody

    • 靶点

      ERN1

    • 浓度

      1mg/ml

    • 应用范围

      IF, IHC-Fr, IHC-P

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb157705

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      详询

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      详询

    • 规格

      50 ul/100 ul/200 ul

    规格:50 ul产品价格:¥3159.0
    规格:100 ul产品价格:¥4914.0
    规格:200 ul产品价格:¥5850.0
    类别: Antibodies

    产品子类型: Primary Antibody

    描述: IRE1 Rabbit Polyclonal Antibody

    别名: IRE1-BETA; IRE1b; IRE2p; hIRE2p; Ern1; Ire1; mIre1; ERN1_HUMAN; Endoplasmic reticulum-to-nucleus signaling 1; Inositol-requiring protein 1 (hIRE1p); Ire1-alpha (IRE1a); ERN1_MOUSE; Inositol-requiring protein 1; endoplasmic reticulum to nucleus signaling 1; ER to nucleus signalling 1; inositol-requiring enzyme 1; Serine/threonine-protein kinase/endoribonuclease IRE1

    应用稀释比例: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    免疫原: KLH conjugated synthetic peptide derived from human IRE1a (252-260/977aa)

    分子量: 135 kDa

    预测反应性: Bovine, Canine, Equine, Porcine, Rabbit

    防腐剂: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化方法: Affinity purified by Protein A

    研究领域: Kinases

    UniProt ID: O75460

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Blank control: Raji. Primary Antibody (green line): Rabbit Anti-IRE1 antibody (orb157705), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Fixative-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IRE1) Polyclonal Antibody, Unconjugated (orb157705) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片3
    Sample: Lane 1: Human Raji cell lysates, Lane 2: Human HeLa cell lysates, Primary: Anti-IRE1 (orb157705) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 105 kDa, Observed band size: 135 kDa.

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