类别: Antibodies
产品子类型: Primary Antibody
描述: DBPA Rabbit Polyclonal Antibody
别名: CSDA; CSDA1; DBPA; ZONAB; Dpba; MSY3; MSY4; Yb2; oxyR; YBOX3_HUMAN; YBX3; Cold shock domain-containing protein A; DNA-binding protein A; Single-strand DNA-binding protein NF-GMB; Q61478_MOUSE; Q68G78_MOUSE; Q8BPG0_MOUSE; YBOX3_MOUSE; Y-box protein 3; Q3KRC7_RAT; YBOX3_RAT; Muscle Y-box protein YB2; RYB-A; Y-box-binding protein A; Y-box binding protein 3; cold shock domain protein A; cold-shock domain containing A1
应用稀释比例: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
免疫原: KLH conjugated synthetic peptide derived from human DBPA/CSDA1 (165-270/372aa)
分子量: 40 kDa
预测反应性: Bovine, Canine, Porcine, Rabbit, Sheep
防腐剂: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化方法: Affinity purified by Protein A
研究领域: Signal Transduction
UniProt ID: P16989
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
Fixative-fixed, paraffin embedded (human heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (orb156545) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (Human kidney carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (orb156545) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (orb156545) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (human skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (orb156545) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (mouse heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (orb156545) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (orb156545) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (mouse skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (orb156545) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (orb156545) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (orb156545) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (orb156545) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (orb156545) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (orb156545) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Mouse Muscle tissue lysates, Lane 2: Rat Muscle tissue lysates, Primary: Anti-DBPA (orb156545) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40 kDa, Observed band size: 40 kDa.
Tissue/Cell: human gastric carcinoma, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-DBPA/CSDA1 Polyclonal Antibody, Unconjugated (orb156545) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: mouse embryo tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-DBPA/CSDA1 Polyclonal Antibody, Unconjugated (orb156545) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: mouse heart tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-DBPA/CSDA1 Polyclonal Antibody, Unconjugated (orb156545) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.